The Gotham River is a river that separates Gotham City from the mainland. It is the main water source for Gotham City and its inhabitants.
Later, the Gotham River was used as an incubator for a number of experimental seeds created by Poison Ivy. Thanks to the sunlight and the algae on the river, the seeds transformed into plant-men and they crawled out of the river to join their creator.
On another winter, the Gotham City Police Department dragged out of the waters a man encased in solid ice, unaware that the man inside was Mister Freeze and that he had chosen the cold waters to preserve his frosty shell while he hibernated.
- On the No Man's Land map, the Gotham River was the river separating the entire city from the mainland, but in the Nolanverse and New 52 redesigns, it is the river separating the south island from the rest of the city.