- Justice League of America
- Wonder Woman
- Green Lantern
- Green Arrow (Cameo)
- Zatanna (Cameo)
- Black Vulcan (Cameo)
- Red Tornado (Cameo)
- Apache Chief (Cameo)
- Martian Manhunter
- Swamp Thing
- Stargirl (Cameo)
- El Dorado (Cameo)
- Samurai (Cameo)
- Doctor Mid-Nite (Cameo)
- Booster Gold (Cameo)
- Wildcat (Dies)
- The Nerd
- Green Lantern Corps
- Lois Lane
- Zoo Crew
- Abigail Arcane
- Jimmy Olsen
- Legion of Doom
- Lex Luthor
- Toyman (Dies)
- The Humping Robot
- Deathstroke (Cameo)
- Solomon Grundy/Gruesday
- Mister Freeze
- Captain Cold
- Black Manta
- Bizarro (Cameo)
- Glenn (Dies)
- Mirror Master
- Mister Banjo
- Catwoman (Cameo)
- Harley Quinn (Cameo)
- General Zod (Mentioned only)
- The Punctuation Posse
- The Exclaimer
- The Comma
- The Quotation Marks
- The Robot Chicken (Cameo)
- The Mad Scientist (Cameo)
- Composite Santa Claus (Cameo)
- The Gummy Bear (Cameo)
- The Unicorn (Cameo)
- Bitch Pudding (Cameo)
- Harry Potter (Mentioned only)
- Snow Job (Mentioned only)
- Selena Gomez (The Nerd's ring construct)
- Atom Smasher (Cameo)
- Gotham City
- Apokolips (Cameo)
- Fortress of Solitude
- Atlantis (Mentioned only)
- Hall of Doom
- Wonder Woman's Invisible Plane (Mentioned only)
This 22-minute special is broken up into many different sketches.
You Can't Fly
Superman, Green Lantern, and Wonder Woman prank Aquaman.
As Batman is about to foil a bank robbery, Bane walks up behind him and breaks his back.
Real Characters from the DC Universe Pt. 1
B'Dg, a squirrel-like Green Lantern, is the first character featured in this series that highlights ridiculous characters from the DC Universe.
The Super Kiss
Superman abuses his power to take away a person's memory.
Two-Face uses a public restroom.
Mr. Freeze, Captain Cold, Icicle, and Chillblaine attempt to steal a diamond from a museum.
Funeral in Earth-C
Superman, Batman, and Green Lantern attend Captain Carrot's funeral, but Green Lantern can't keep but laughing at Earth-C's cartoonish inhabitants.
Swamp Thing's hygiene is questioned.
That's Bane! Again!
After fully recovering from his injuries, Bane yet again breaks Batman's back.
Real Characters from the DC Universe 2
The second character featured is Firestorm, who's offended that he's in this.
Out to Score
Flash, Batman, Green Lantern, Aquaman, and Superman go to a bar to hit the scene to meet some ladies. Aquaman is humiliated.
The Legion of Doom draws for this year's Secret Santa, and complains about a co-worker's odor.
The Nerd becomes joins the Green Lantern Corps, and trains with Kilowog on Oa. NOTE: Unlike the rest of the episode, the Hal Jordan featured in this segment is based off the Ryan Reynolds version.
Luthor is impressed by his newest weapon, but quickly finds a flaw in its design.
A criminal shoots Superman.
The Punctuation Posse
The Riddler forms his own gang when the Legion of Doom shoots down his ideas.
That's Bane! Thrice!
Batman comes back to the Justice League after recovering from his last encounter with Bane, only for the villain to break his back a third time as his comrades look on.
Mirror Master and the Riddler pressure Sinestro into shaving his mustache, with disastrous results.
That Is It!
Aquaman is fed up with the rest of the Justice League disrespecting him.
Real Characters from the DC Universe 3
Mister Banjo is the third character featured in this segment, to which Firestorm reacts violently.
Aquaman joins the Legion of Doom to get back at the Justice League.
Born on a Tuesday
Solomon Grundy finds out his real identity.
Aquaman, now secretly a member of the Legion of Doom, brings a cake filled with supervillains to the Hall of Justice for Robin's Bar Mitzvah, which turns out to be a cover for a surprise party for Aquaman. The villains breaks out of the cake, leading to an all-out brawl between the Legion of Doom and the Justice League, and Aquaman much decide where his loyalties reside.
Someone has died.
- Abraham Benrubi as Icicle, Solomon Grundy, Kilowog, and Cyborg
- Alex Borstein as Wonder Woman and Giganta
- Nathan Fillion as Green Lantern and Mister Freeze
- Megan Fox as Lois Lane
- Clare Grant as Ice, Abigail Arcane, and Cheetah
- Seth Green as The Nerd, Batman, Robin, Aquaman, Penguin, Little Cheese, Swamp Thing, Martian Manhunter, Toyman, Green Arrow, Scarecrow, Abin Sur, Ganthet, Grodd, Hawkman, Jimmy Olsen, and various others
- Neil Patrick Harris as Two-Face and Black Manta
- Breckin Meyer as Superman and Mirror Master
- Alfred Molina as Lex Luthor and Firestorm
- Aaron Paul as Mister Banjo and Glenn
- Paul Reubens as Riddler
- Tom Root as Captain Cold
- Matthew Senreich as Flash, Brainiac, and Chillblaine
- Kevin Shinick as the Narrator
- Tara Strong as Harley Quinn and Selena Gomez
- Zeb Wells as Sinestro and B'dg
- This TV Special was made in collaboration with DC Comics.
- Most of the characters that appear in this special are an amalgam of the versions of their characters from various sources: Earth-One, New Earth, the Super Friends cartoon, and various movies.
- No trivia.
- Characters from Robot Chicken DC Comics Special
- Other things related to Robot Chicken DC Comics Special
- Movie Gallery: Robot Chicken DC Comics Special